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Achievements

2024

Chih-Hsuan Lee, Jing-Ee Saw, Eric H-L Chen, Chun-Hsiung Wang, Takayuki Uchihashi, Rita P-Y Chen*, "The positively charged cluster in the N-terminal disordered region may affect prion protein misfolding: Cryo-EM structure of hamster PrP (23-144) fibrils", Journal of Molecular Biology  436, 168576 (2024). DOI: 10.1016/j.jmb.2024.168576

Yuki Miyata, Feng-Yueh Chan, Takayuki Uchihashi, Takaharu Okajima*, "Compressed sensing reconstruction of cell mechanical images obtained from atomic force microscopy", Japanese Journal of Applied Physics  63, 04SP46 (2024). DOI: 10.35848/1347-4065/ad34de

Saeko Yanaka, Atsuji Kodama, Shigetaka Nishiguchi, Rina Hiramine, Jiana Shen, Pornthip Boonsri, Duckyong Sung, Yukiko Isono, Hirokazu Yagi, Yohei Miyanoiri, Takayuki Uchihashi, Koichi Kato*, "Identification of potential C1-binding sites in the immunoglobulin CL domains", International Immunology  (in press) (2024). DOI: 10.1093/intimm/dxae017

Miki Kinoshita, Tohru Minamino*, Takayuki Uchihashi, Keiichi Namba, "FliH and FliI help FlhA bring strict order to flagellar protein export in Salmonella", Communications Biology  7, 366 (2024). DOI: 10.1038/s42003-024-06081-0

Yuki Miyata, Feng-Yueh Chan, Takayuki Uchihashi, Takaharu Okajima*, "Compressed sensing reconstruction of cell mechanical images obtained from atomic force microscopy", Japanese Journal of Applied Physics  63, 04SP46 (2024). DOI: 10.35848/1347-4065/ad34de

Takayuki Uchihashi*,Yuichiro Nishizawa, "Application of high-speed atomic force microscopy in visualizing the dynamics of synthetic polymers", Japanese Journal of Applied Physics  63, 040803 (2024). DOI: 10.35848/1347-4065/ad33f2

Takayuki Uchihashi, Felix Rico, Jean‐luc Pellequer, "Tenth International AFMBioMed Conference on AFM in Life Sciences and Medicine, August 30–September 2, 2022, Nagoya‐Okasaki, Japan in memoriam of Pierre Parot (1950–2023)", Journal of Molecular Recognition (in press),  (2024). DOI: 10.1002/jmr.3077

Keishi Yang​, Feng-Yueh Chan, Hiroki Watanabe, Shingo Yoshioka, Yasushi Inouye, Takayuki Uchihashi, Hidekazu Ishitobi, Prabhat Verma, Takayuki Umakoshi*, "In Situ Real-Time Observation of Photoinduced Nanoscale Azo-Polymer Motions Using High-Speed Atomic Force Microscopy Combined with an Inverted Optical Microscope", Nano Letters 24, 2805-2811 (2024). DOI: 10.1021/acs.nanolett.3c04877

Christian Ganser*, Takayuki Uchihashi*, "Measuring mechanical properties with high-speed atomic force microscopy", Microscopy  73, 14-21 (2024). DOI: 10.1093/jmicro/dfad051

Kentaro Mannen, Takashi Nagata, Andrey Rozenberg, Masae Konno, María del Carmen Marín, Reza Bagherzadeh, Oded Béjà, Takayuki Uchihashi, Keiichi Inoue*, "Multiple Roles of a Conserved Glutamate Residue for Unique Biophysical Properties in a New Group of Microbial Rhodopsins Homologous to TAT Rhodopsin", Journal of Molecular Biology 43, 14-21 (2024). DOI: 10.1093/jmicro/dfad051

Saeko Yanaka, Hiroki Watanabe, Rina Yogo, Mesayamas Kongsema, Sachiko Kondo, Hirokazu Yagi, Takayuki Uchihashi*, Koichi Kato*, "Quantitative Analysis of Therapeutic Antibody Interactions with Fcγ Receptors Using High-Speed Atomic Force Microscopy", Biological and Pharmaceutical Bulletin 47, 334-338 (2024). DOI: 10.1248/bpb.b23-00751

2024

2023

Yoshiko Tanaka, Takayuki Uchihashi, Akihiko Nakamura*, "Product inhibition slow down the moving velocity of processive chitinase and sliding-intermediate state blocks re-binding of product", Archives of Biochemistry and Biophysics 47,  334-338 (2024). DOI: 10.1016/j.abb.2023.109854

Carol Cho, Christian Ganser, Takayuki Uchihashi, Koichi Kato & Ji-Joon Song*"Structure of the human ATAD2 AAA+ histone chaperone reveals mechanism of regulation and inter-subunit communication", Communications Biology 6, Article number: 993 (2023). DOI: 10.1038/s42003-023-05373-1

Eric H.-L. Chen, Chun-Hsiung Wang, Yi-Ting Liao, Feng-Yueh Chan, Yui Kanaoka, Takayuki Uchihashi, Koichi Kato, Longsheng Lai, Yi-Wei Chang, Meng-Chiao Ho* and Rita P.-Y. Chen*"Visualizing the membrane disruption action of antimicrobial peptides by cryo-electron tomography", Nature Communications 14, 5464 (2023). DOI: 10.1038/s41467-023-41156-2

Yuki Konishi, Masafumi Minoshima, Kohei Fujihara, Takayuki Uchihashi and Kazuya Kikuchi*, "Elastic Polymer Coated Nanoparticles with Fast Clearance for 19F MR Imaging", Angewandte Chemie - International Edition 62,  e2023085 (2023). DOI: 10.1002/ange.202308565

Maho Yagi-Utsumi, Haruko Miura, Christian Ganser, Hiroki Watanabe, Methanee Hiranyakorn, Tadashi Satoh, Takayuki Uchihashi, Koichi Kato, Kei-ichi Okazaki* and Kazuhiro Aoki*, "Molecular Design of FRET Probes Based on Domain Rearrangement of Protein Disulfide Isomerase for Monitoring Intracellular Redox Status", International Journal of Molecular Sciences 24, 12865 (2023). DOI: 10.3390/ijms241612865

Xuguang Jiang, Tadayuki Ogawa, Kento Yonezawa, Nobutaka Shimizu, Sotaro Ichinose, Takayuki Uchihashi, Wataru Nagaike, Toshio Moriya, Naruhiko Adachi, Masato Kawasaki, Naoshi Dohmae, Toshiya Senda, Nobutaka Hirokawa*, "The two-step cargo recognition mechanism of heterotrimeric kinesin", EMBO Reports, e5684. DOI: 10.15252/embr.202356864

Yu-Chun Chien, Yong-Sheng Wang, Deepa Sridharan, Chu-Wei Kuo, Chih-Ta Chien, Takayuki Uchihashi, Koichi Kato, Takashi Angata, Tzu-Ching Meng, Shang-Te Danny Hsu, and Kay-Hooi Khoo*, "High Density of N- and O-Glycosylation Shields and Defines the Structural Dynamics of the Intrinsically Disordered Ectodomain of Receptor-type Protein Tyrosine Phosphatase Alpha", JACS Au 3, 1864-1875 (2023). DOI: 10.1021/jacsau.3c00124

Kanji Takahashi, Tatsuro Nishikino, Hiroki Kajino, Seiji Kojima, Takayuki Uchihashi*, Michio Homma*, "Ring formation by Vibrio fusion protein composed of FliF and FliG, MS-ring and C-ring component of bacterial flagellar motor in membrane", Biophysics and Physicobiology  20, e200028 (2023). DOI: 10.2142/biophysico.bppb-v20.0028

Norihiko Sasaki, Jun Kikkawa, Yoshiki Ishii, Takayuki Uchihashi, Hitomi Imamura, Masayuki Takeuchi, Kazunori Sugiyasu*, "Multistep, site-selective noncovalent synthesis of two-dimensional block supramolecular polymers", Nature Chemistry 15, 922-929 (2023). DOI: 10.1038/s41557-023-01216-y

Yuichiro Nishizawa, Hiroki Yokoi, Takayuki Uchihashi*, Daisuke Suzuki*, "Single microgel degradation governed by heterogeneous nanostructures", Soft Matters 19, 5068-5075 (2023). DOI: 10.1039/D3SM00216K

Shigetaka Nishiguchi*, Rinshi Kasai*, Takayuki Uchihashi*, "Antiparallel dimer structure of CELSR cadherin in solution revealed by high-speed-atomic force microscopy", Proceedings of the National Academy of Sciences of the United States of America 120, e2302047120 (2023). DOI: 10.1073/pnas.2302047120

Toshinobu Nakajo, Shinpei Kusaka, Haruka Hiraoka, Kohei Nomura, Noriaki Matsubara, Rintaro Baba, Yuki Yoshida, Kosuke Nakamoto, Masakazu Honma, Hiroaki Iguchi, Takayuki Uchihashi, Hiroshi Abe and Ryotaro Matsuda*, "Creation of Single Molecular Conjugate of Metal-organic Cage and DNA", Chemical Communications 59, 4974-4977 (2023). DOI: doi.org/10.1039/D3CC00460K

Takumi Watanabe, Haruka Minato, Yuma Sasaki, Seina Hiroshige, Koki Sano, Takeshi Wakiya, Yuichiro Nishizawa, Takayuki Uchihashi, Takuma Kureha, Mitsuhiro Shibayama, Toshikazu Takata, and Daisuke Suzuki*, "Closed-loop recycling of microparticle-based polymers", Green Chemistry 25, 3418-3424 (2023). DOI: 10.1039/D3GC00090G

Julian Wong Soon ,Koji Oohora*, Takayuki Uchihashi* and Takashi Hayashii*, "Disulfide Bond-Mediated Oligomerization of a Green Fluorescent Protein in Solution", Chemistry Letters 52, 105-109 (2023). DOI: 10.1246/cl.220495

Yoshitaka Matsuo*, Takayuki Uchihashi and Toshifumi Inada*, "Decoding of the ubiquitin code for clearance of colliding ribosomes by the RQT complex", Nature Communications 14, 79 (2023). DOI: 10.1038/s41467-022-35608-4

Rubaya Rashid, Christian Ganser, Mousumi Akter, Syeda Rubaiya Nasrin, Arif Md. Rashedul Kabir, Kazuki Sada, Takayuki Uchihashi, and Akira Kakugo*, "3D structure of ring-shaped microtubule swarms revealed by high-speed atomic force microscopy", Chemistry Letters 52, 100-104 (2023). DOI: 10.1246/cl.220491

Youfeng Yue*, Yoshiko Yokota, Takayuki Uchihashi, "Biosynthesis of highly branched gold nanoparticles through structural engineering of fatty acids", iScience 20, 105864 (2023). DOI:10.1016/j.isci.2022.105864 

2023

2022

Taku Uchiyama, Takayuki Uchihashi, Takuya Ishida, Akihiko Nakamura, Josh Vermaas, Michael F. Crowley, Masahiro Samejima, Gregg T. Beckham, Kiyohiko Igarashi*, "Lytic polysaccharide monooxygenase increases cellobiohydrolases activity by promoting decrystallization of cellulose surface", Science Advances 8, eade5155 (2022), DOI:10.1126/sciadv.ade5155

Taku Uchiyama, Takayuki Uchihashi, Takuya Ishida, Akihiko Nakamura, Josh Vermaas, Michael F. Crowley, Masahiro Samejima, Gregg T. Beckham, Kiyohiko Igarashi*, "Lytic polysaccharide monooxygenase increases cellobiohydrolases activity by promoting decrystallization of cellulose surface", Science Advances 8, eade5155 (2022), DOI:10.1126/sciadv.ade5155.

Feng-Yueh Chan, Ryo Kurosaki, Christian Ganser, Tetsuya Takeda, and Takayuki Uchihashi*, "Tip-scan high-speed atomic force microscopy with a uniaxial substrate stretching device for studying dynamics of biomolecules under mechanical stress", Review of Scientific Instruments  93, 113703 (2022). DOI:10.1063/5.0111017

Mizuki Sumikawa, Rei Abe-Yoshizumi, Takayuki Uchihashi, and Hideki Kandori*, "Mechanism of the Irreversible Transition from Pentamer to Monomer at pH 2 in a Blue Proteorhodopsin", Biochemistry 61, 936-1944 (2022). DOI: 10.1021/acs.biochem.2c00328

Shigetaka Nishiguchi*, Tadaomi Furuta, and Takayuki Uchihashi*, "Multiple dimeric structures and strand-swap dimerization of E-cadherin in solution visualized by high-speed atomic force microscopy", Proceedings of the National Academy of Sciences of the United States of America  119,  e2208067119 (2022). DOI: 10.1073/pnas.2208067119

Eriko Hamasaki, Natsuki Wakita, Hiroki Yasuoka, Hikaru Nagaoka, Masayuki Morita, Eizo Takashima, Takayuki Uchihashi, Tetsuya Takeda, Tadashi Abe, Ji-Won Lee, Tadahiro Iimura, Moin A Saleem, Naohisa Ogo, Akira Asai, Akihiro Narita, Kohji Takei* and Hiroshi Yamada*, "The Lipid-Binding Defective Dynamin 2 Mutant in Charcot-Marie-Tooth Disease Impairs Proper Actin Bundling and Actin Organization in Glomerular Podocytes", Frontiers in Cell and Developmental Biology 10, 8845092090 (2022). DOI: 10.3389/fcell.2022.884509

Saeko Yanaka#, Shigetaka Nishiguchi#, Rina Yogo#, Hiroki Watanabe, Jiana Shen, Hirokazu Yagi, Takayuki Uchihashi*, and Koichi Kato*, "Quantitative Visualization of the Interaction between Complement Component C1 and Immunoglobulin G: The Effect of CH1 Domain Deletion", International Journal of Molecular Sciences, 23, 2090 (2022). (#equal contribution) DOI: 10.3390/ijms23042090 

Kosuke Kikuchi, Tatsuya Fukuyama, Takayuki Uchihashi, Tadaomi Furuta, Yusuke T. Maeda, and Takafumi Ueno*, "Protein Needles Designed to Self-Assemble through Needle Tip Engineering", Small, 18, 2106401 (2022). DOI: 10.1002/smll.202106401

Yutaro Yamashita, Shohei Tashiro*, Yoshiki Ishii,Takayuki Uchihashi, Nobuyuki Matsushita, Ryou Kubota, and Mitsuhiko Shionoya*, "Shape-selective one-step synthesis of branched gold nanoparticles on the crystal surface of redox-active PdII-macrocycles", Dalton Transactions, 51, 1318-1324 (2022). DOI: 10.1039/D1DT03973C

2022

2021

Mizuki Sumikawa, Rei Abe-Yoshizumi, Takayuki Uchihashi, and Hideki Kandori *, "Molecular Origin of the Anomalous pH Effect in Blue Proteorhodopsin", Journal of Physical Chemistry Letters 12, 12225-12229 (2021). DOI: 10.1021 / acs.jpclett .1c03355

Masaru Yamanaka, Tsuyoshi Mashima, Michio Ogihara, Mei Okamoto, Takayuki Uchihashi, and Shun Hirota *, "Construction of ferritin hydrogels utilizing subunit–subunit interactions", PLoS ONE 16, e0259052 (2021). DOI: 10.1371 / journal.pone.0259052

Maho Yagi-Utsumi, Kazuhiro Aoki, Hiroki Watanabe, Chihong Song, Seiji Nishimura, Tadashi Satoh, Saeko Yanaka, Christian Ganser, Sae Tanaka, Vincent Schnapka, Ean Wai Goh, Yuji Furutani, Kazuyoshi Murata, Takayuki Uchihashi, Kazuharu Arakawa & Koichi Kato *, "Desiccation-induced fibrous condensation of CAHS protein from an anhydrobiotic tardigrade", Scientific Reports 11, 21328 (2021). DOI: 10.1038 / s41598-021-00724-6

Syeda Ruvaiya Nasrin #, Christian Ganser #, Seiji Nishikawa, Arif MD. Rashedul Kabir, Kazuki Sada, Takefumi Yamashita, Mitsunori Keguchi, Takayuki Uchihashi, Henry Hess, and Akira Kakugo *, "Deformation of microtubules regulates translocation dynamics of kinesin", Science Advances 7, eabf2211 (2021) (#equal contribution). DOI: 10.1126 / sciadv.abf2211.

Kazuhisa Miyazawa, Satoru G. Itoh, Hiroki Watanabe, Takayuki Uchihashi, Saeko Yanaka, Maho Yagi-Utsumi, Koichi Kato, Kazuharu Arakawa, and Hisashi Okumura *, "Tardigrade Secretory-Abundant Heat-Soluble Protein Has a Flexible β-Barrel Structure in Solution and Keeps This Structure in Dehydration ", Journal of Physical Chemistry B, 125, 9145–9154 (2021). DOI: doi.org/10.1021/acs.jpcb.1c04850.

Bhaskar Dasgupta, Osamu Miyashita, Takayuki Uchihashi and Florence Tama *, "Reconstruction of Three-Dimensional Conformations of Bacterial ClpB from High-Speed Atomic-Force-Microscopy Images", Frontiers in in Molecular Biosciences: Biological Modeling and Simulation 8, 704274 (2021) ). DOI: 10.3389 / fmolb.2021.704274.

Ayuko Sakane *, Taka-aki Yano, Takayuki Uchihashi, Kazuki Horikawa, Yusuke Hara, Issei Imoto, Shusaku Kurisu, Hiroshi Yamada, Kohji Takei and Takuya Sasaki *, "JRAB / MICAL-L2 undergoes liquid–liquid phase separation to form tubular recycling endosomes ", Communications Biology 4, 551 (2021). DOI: 10.1038 / s42003-021-02080-7.

Yuma Sasaki, Seina Hiroshige, Masaya Takizawa, Yuichiro Nishizawa, Takayuki Uchihashi, Haruka Minato and Daisuke Suzuki *, "Non-close-packed arrangement of soft elastomer microspheres on solid conveyed", RSC Advances 11, 14562-14567 (2021). DOI : 10.1039 / D1RA02688G.

Yuichiro Nishizawa, Haruka Minato, Takumi Inui, Ikuma Saito, Takuma Kureha, Mitsuhiro Shibayama, Takayuki Uchihashi * and Daisuke Suzuki *, "Nanostructure and thermoresponsiveness of poly (N-isopropyl methamine)-based hydrogel microspheres prepared via aqueous free radical precipitation polymerization" , RSC Advances 11, 13130-13137 (2021). DOI: 10.1039 / D1RA01650D.

Yuichiro Nishizawa, Haruka Minato, Takumi Inui, Takayuki Uchihashi *, Daisuke Suzuki *, “Nanostructures, Thermoresponsiveness, and Assembly Mechanism of Hydrogel Microspheres during Aqueous Free-Radical Precipitation Polymerization”, Langmuir 37 151-159 (2021). DOI: 10.1021 / acs.langmuir.0c02654.

Junso Fujita, Shogo Sugiyama, Haruna Terakado, Maho Miyazaki, Mayuki Ozawa, Nanami Ueda, Natsuko Kuroda, Shun-ichi Tanaka, Takuya Yoshizawa, Takayuki Uchihashi * and Hiroyoshi Matsumura *, "Dynamic Assembly / Disassembly of Staphylococcus aureus FtsZ Visualized by High- Speed Atomic Force Microscopy ", International Journal of Molecular Sciences 22, 1697 (2021). DOI: 10.3390 / ijms22041697.

2021

2020

Basudev Maity, Zhipeng Li, Kento Niwase, Christian Ganser, Tadaomi Furuta, Takayuki Uchihashi, Diannan Lu, Takafumi Ueno *, "Single-molecule level dynamic observation of disassembly of the apo-ferritin cage in solution", Physical Chemistry Chemical Physics 22, 18562-18572 (2020). DOI: 10.1039 / D0CP02069A.

Hassan Hakimi, Thomas J Templeton, Miako Sakaguchi, Junya Yamagishi, Shinya Miyazaki, Kazuhide Yahata, Takayuki Uchihashi, Shin-ichiro Kawazu, Osamu Kaneko, Masahito Asada *, "Novel Babesia bovis exported proteins that modify properties of infected red blood cells", PLoS pathogens 16, e1008917 (2020). DOI: 110.1371 / journal.ppat.1008917.

Tatsuya Ikuta, Wataru Shihoya, Masahiro Sugiura, Kazuho Yoshida, Masahito Watari, Takaya Tokano, Keitaro Yamashita, Kota Katayama, Satoshi P. Tsunoda, Takayuki Uchihashi, Hideki Kandori * and Osamu Nureki *, "Structural insights into the mechanism of rhodopsin , Nature Communications 11,5605 (2020). DOI: 10.1038 / s41467-020-19376-7.

Taku Uchiyama #, Takayuki Uchihashi #, Akihiko Nakamurad, Hiroki Watanabec, Satoshi Kanekoe, Masahiro Samejimaa, and Kiyohiko Igarashi *, "Convergent evolution of processivity in bacterial and fungal cellulases", Proceedings of the National Academy of Sciences of the United States of America , 117, 19896-19903 (2020). DOI: 10.1073 / pnas.2011366117. (#equal contribution)

Nesreen Hamad, Hiroki Watanabe, Takayuki Uchihashi, Riki Kurokawa, Takashi Nagata, and Masato Katahira *, "Direct visualization of the conformational change of FUS / TLS upon binding to promoter-associated non-coding RNA", Chemical Communications 56, 9134-9137 (2020). DOI: 10.1039 / D0CC03776A.

Takaya Tokano, Yuki Kato, Shogo Sugiyama, Takayuki Uchihashi *, and Takumi Noguchi *, "Structural Dynamics of a Protein Domain Relevant to the Water-Oxidizing Complex in Photosystem II as Visualized by High-Speed Atomic Force Microscopy", The Journal of Physical Chemistry B 124, 5847-5857 (2020). DOI: 10.1021 / acs.jpcb.0c03892.

Hiroyuki Terashima, Keiichi Hirano, Yuna Inoue, Takaya Tokano, Akihiro Kawamoto, Takayuki Kato, Erika Yamaguchi, Keiichi Namba, Takayuki Uchihashi, Seiji Kojima and Michio Homma *, "Assembly mechanism of a supramolecular MS-ring complex to initiate bacterial flagellar biogenesis in Vibrio species ", Journal of Bacteriology 202, e00236-20 (2020). DOI: 10.1128 / JB.00236-20.

Haruka Minato, Yuichiro Nishizawa, Takayuki Uchihashi * and Disuke Suzuki *, "Thermoresponsive structural changes of single poly (N-isopropyl acrylamide) hydrogel microspheres under densely packed conditions on a solid substrate", Polymer Journal 52, 1137-1141 (2020). DOI: 110.1038 / s41428-020-0372-3.

Keiichi Inoue *, Satoshi P. Tsunoda, Manish Singh, Sahoko Tomida, Shoko Hososhima, Masae Konno, Ryoko Nakamura, Hiroki Watanabe, Paul-Adrian Bulzu, Horia L. Banciu, Adrian-Ştefan Andrei, Takayuki Uchihashi, Rohit Ghai, Oded Béjà and Hideki Kandori *, "Schizorhodopsins: A family of rhodopsins from Asgard archaea that function as light-driven inward H + pumps", Science Advances 6, eaaz2441 (2020). DOI: 10.1126 / sciadv.aaz2441.

Hiroki Konno, Takahiro Watanabe-Nakayama, Takayuki Uchihashi, Momoko Okuda, Liwen Zhu, Noriyuki Kodera, Yousuke Kikuchi, Toshio Ando and Hideki Taguchi *, "Dynamics of oligomer and amyloid fibril formation by yeast prion Sup35 observed by high-speed atomic force microscopy ", Proceedings of the National Academy of Sciences of the United States of America 117, 7831-7836 (2020). DOI: 110.1073/pnas.1916452117.

Takafumi Ueno *, Kento Niwase, Daisho Tsubokawa, Kosuke Kikuchi, Natsumi Takai, Tadaomi Furuta, Ryuji Kawano, Takayuki Uchihashi, "Dynamic behavior of an artificial protein needle contacting a membrane observed by high-speed atomic force microscopy", Nanoscale 12, 8166 -8173 (2020). DOI: 10.1039 / D0NR01121E.

Takayuki Uchihashi * and Christian Ganser *, "Recent advances in bioimaging with high-speed atomic force microscopy", Biophysical Reviews12, 363-369 (2020). DOI: 10.1007 / s12551-020-00670-z.

Akasit Visootsat, Akihiko Nakamura Paul Vignon, Hiroki Watanabe, Takayuki Uchihashi and Ryota Iino *, "Single-molecule imaging analysis reveals the mechanism of a high-catalytic-activity mutant of chitinase A from Serratia marcescens", Journal of Biological Chemistry 295, 1915 -1925 (2020). DOI: 10.1074 / jbc.RA119.012078.

Shota Hirayama, Koji Oohora *, Takayuki Uchihashi * and Takashi Hayashi *, "A Thermoresponsive Micellar Assembly Constructed from a Hexameric Hemoprotein Modified with Poly (N-isopropyl PASSWORD) toward an Artificial Light-harvesting System", Journal of American Chemical Society 142, 1822 -1831 (2020). DOI: 10.1021 / jacs.9b10080.

Koji Oohora *, Shota Hirayama, Takayuki Uchihashi * and Takashi Hayashi *, "Construction of a Hexameric Hemoprotein Sheet and Direct Observation of Dynamic Process of its Formation", Chemistry Letters 49, 186-190 (2020). DOI: 10.1246 / cl. 190855.

Maho Yagi-Utsumi, Arunima Sikdar, Chihong Song, Jimin Park, Rintaro Inoue, Hiroki Watanabe, Raymond N. Burton-Smith, Toshiya Kozai, Tatsuya Suzuki, Atsuji Kodama, Kentaro Ishii, Hirokazu Yagi, Tadashi Satoh, Susumu Uchiyama, Takayuki Uchih , Keehyoung Joo, Jooyoung Lee, Masaaki Sugiyama, Kazuyoshi Murata and Koichi Kato, "Supramolecular tholos-like architecture composed by archaeal proteins without functional annotation", Scientific Reports10, 1540 (2020). DOI: 10.1038 / s41598-020-58371-2 ..

Hisashi Tatebe, Chew Theng Lim, Hiroki Konno, Kazuhiro Shiozaki, Akira Shinohara, Takayuki Uchihashi * and Asako Furukohri *, "Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge", Nature Communications 11, Article number: 370 (2020) ). DOI: 10.1038 / s41467-019-14025-0.

Saeko Yanaka #, Rina Yogo #, Hiroki Watanab #, Yuki Taniguchi, Tadashi Satoh, Naoko Komura, Hiromune Ando, Hirokazu Yagi, Nobuhiro Yuki, Takayuki Uchihashi *, Koichi Kato *, "On-Membrane Dynamic Interplay between Anti-GM1 IgG Antibodies and Complement Component C1q ", International Journal of Molecular Sciences, 21, 147 (2020). DOI: 10.3390 / ijms21010147. (#equal contribution)

Takayuki Umakoshi, Shingo Fukuda, Ryota Iino, Takayuki Uchihashi and Toshio Ando *, "High-speed near-field fluorescence microscopy combined with high-speed atomic force microscopy for biological studies", Biochimica et Biophysica Acta (BBA) --General Subjects 1864, 129325 (2020), DOI: 10.1016 / j.bbagen.2019.03.011.

2020

2019

Carol Cho, Juwon Jang, Yujin Kang, Hiroki Watanabe, Takayuki Uchihashi, Seung Joong Kim, Koichi Kato, Ja Yil Lee * and Ji-Joon Song *, "Structural basis of nucleosome assembly by the Abo1 AAA + ATPase histone chaperone", Nature Communications 10, Article number: 5764 (2019). DOI: 10.1038 / s41467-019-13743-9.

Wataru Shihoya, Keiichi Inoue, Manish Singh, Masae Konno, Shoko Hososhima, Keitaro Yamashita, Kento Ikeda, Akimitsu Higuchi, Tamaki Izume, Sae Okazaki, Masanori Hashimoto, Ritsu Mizutori, Sahoko Tomida, Yumeka Yamauchi, Rei Abe-Yoshizumi, Kota Satoshi P. Tsunoda, Mikihiro Shibata, Yuji Furutani, Alina Pushkarev, Oded Béjà, Takayuki Uchihashi, Hideki Kandori * and Osamu Nureki *, "Crystal structure of heliorhodopsin", Nature 574, 132-136 (2019). DOI: 10.1038 / s41586 -019-1604-6.

Rina Yogo, Yuki Yamaguchi, Hiroki Watanabe, Hirokazu Yagi, Tadashi Satoh, Mahito Nakanishi, Masayoshi Onitsuka, Takeshi Omasa, Mari Shimada, Takahiro Maruno, Tetsuo Torisu, Shio Watanabe, Daisuke Higo, Takayuki Uchihashi, Saeko Yanaka, Susumu Uchiyama and Koichi *, "The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III", Scientific Reports 9, 11957 (2019). DOI: 10.1038 / s41598-019-48323-w.

Shusuke Matsui, Kensuke Hosho, Haruka Minato, Takayuki Uchihashi * and Daisuke Suzuki *, "Protein Uptake into Individual Hydrogel Microspheres Visualized by High-Speed Atomic Force Microscopy", Chemical Communications 55, 10064-10067 (2019). DOI: 10.1039 / C9CC05116C ..

Lei Feng, Hiroki Wantanabe, Paul Molino, Gordon Wallace, Son Lam Phung, Takayuki Uchihashi and Michael Higgins *, "Dynamics of Inter-Molecular Interactions Between Single Aβ42 Oligomeric and Aggregate Species by High-Speed Atomic Force Microscopy", Journal of Molecular Biology 46, 2687-2699 (2019). DOI: 10.1016 / j.jmb.2019.04.044.

Taichiro Sekiguchi, Tadashi Satoh, Eiji Kurimoto, Chihong Son, Toshiya Kozai, Hiroki Watanabe, Kentaro Ishii, Hirokazu Yagi, Saeko Yanaka Susumu Uchiyama, Takayuki Uchihashi, Kazuyoshi Murata and Koichi Kato *, "Mutational and combinatorial control of self-assembling and disassembling of human proteasome α-subunits ", International Journal of Molecular Sciences 20, 2608 (2019). DOI: 0.3390 / ijms20092308.

Yuichiro Nishizawa, Shusuke Matsui, Kenji Urayama, Takuma Kureha, Mitsuhiro Shibayama, Takayuki Uchihashi * and Daisuke Suzuki *, "Non-Thermoresponsive Decanano-sized Domains in Thermoresponsive Hydrogel Microspheres Revealed by Temperature-Controlled High-Speed Atomic Force Microscopy", Angewandte Chemie International Edition 58, 8809-8813 (2019). DOI: 10.1002 / anie.201903483.

Ayumi Sumino # *, Takashi Sumikama #, Takayuki Uchihashi *, Shigetoshi Oiki *, "High-speed AFM reveals accelerated binding of Agitoxin-2 to K + channel by induced-fit", Science Advances 5, eaax0495 (2019). DOI: 10.1126 /sciadv.aax0495. (#equal contribution)

Takaaki Miyamoto, Yugo Hayashi, Keito Yoshida, Hiroki Watanabe, Takayuki Uchihashi, Kento Yonezawa, Nobutaka Shimizu, Hironari Kamikubo, Shun Hirota *, "Construction of a quadrangular tetramer and a cage-like hexamer from three-helix bundle-linked fusion proteins" , ACS Synthetic Biology 8, 1112-1120 (2019). DOI: 10.1021 / acssynbio.9b00019.

Kenshiro Honda, Yuka Sazuka, Kojiro Iizuka, Shusuke Matsui, Takayuki Uchihashi, Takuma Kureha, Mitsuhiro Shibayama, Takumi Watanabe and Daisuke Suzuki *, "Hydrogel Microellipsoids that Form Robust String-like Assemblies at the Air / Water Interface", Angewandte Chemie International Edition 58, 1-6 (2019). DOI: 10.1002 / anie.201901611.

Elena Krayukhina, Masami Yokoyama, Kayoko Kakuhou Hayashihara, Takahiro Maruno, Masanori Noda, Hiroki Watanabe, Takayuki Uchihashi, Susumu Uchiyama *, "An assessment of the ability of submicron- and micron-size silicone oil droplets indropped pre-fillable silicas to invoke early --and late-stage immune responses ", Journal of Pharmaceutical Sciences 108, 2278-2287 (2019). DOI: 10.1016 / j.xphs. 2019.02.002

Mikito Owa, Takayuki Uchihashi, Haru-aki Yanagisawa, Takashi Yamano, Hiro Iguchi, Hideya Fukuzawa, Ken-ichi Wakabayashi, Toshio Ando and Masahide Kikkawa *, "Inner lumen proteins stabilize doublet microtubules in cilia and flagella", Nature Communications 10, Article number: 1143 (2019). DOI: 10.1038 / s41467-019-09051-x.

Shintaro Maruyama, Kano Suzuki, Motonori Imamura, Hikaru Sasaki, Hideyuki Matsunami, Kenji Mizutani, Yasuko Saito, Fabiana L. Imai, Yoshiko Ishizuka-Katsura, Tomomi Kimura-Someya, Mikako Shirouzu, Takayuki Uchihashi, Toshio Ando, Ichiro Yamato and Takeshi Murata *, "Metastable asymmetrical structure of a shaftless V1 motor", Science Advances 5, eaau8149 (2019). DOI: 10.1126 / sciadv.aau8149.

Koji Oohora *, Ryota Kajihara, Nishiki Fujimaki, Takayuki Uchihashi * and Takashi Hayashi *, "A Ring-shaped Hemoprotein Trimer Thermodynamically Controlled by the Supramolecular Heme–Heme Pocket Interaction", Chemical Communications 55, 1544-1547 (2019). DOI: 10.1039 / C8CC09314H.

Christian Ganser * and Takayuki Uchihashi *, "Microtubule self-healing and defect creation investigated by in-line force measurements during high-speed atomic force microscopy", Nanoscale 11, 125-135 (2019). DOI: 10.1039 / C8NR07392A.

Takamitsu Haruyama #, Yasunori Sugano #, Noriyuki Kodera, Takayuki Uchihashi, Toshio Ando, Yoshiki Tanaka, Hiroki Konno *, and Tomoya Tsukazaki *, "Single-unit imaging of membrane protein-embedded nanodiscs from two oriented sides by high-speed atomic force microscopy ", Structure 27, 152-160 (2019). DOI: 10.1016 / j.str.2018.09.005. (#equal contribution)

2019

2018

Tomoya Fukui, Takayuki Uchihashi*,Norihiko Sasaki, Hiroki Watanabe, Masayuki Takeuchi* and Kazunori Sugiyasu*, "Direct observation and manipulation of supramolecular polymerization by using high-speed atomic force microscopy", Angewandte Chemie International Edition 57, 15465-15470 (2018). DOI: 10.1002/anie.201809165

Shusuke Matsui, Yuichiro Nishizawa, Takayuki Uchihashi* and Daisuke Suzuki*, "Monitoring Thermo-responsive Morphological Changes in Individual Hydrogel Microspheres", ACS Omega 3, 10836-10842 (2018). DOI: 10.1021/acsomega.8b01770

Koji Oohora*, Nishiki Fujimaki, Ryota Kajihara, Hiroki Watanabe, Takayuki Uchihashi* and Takashi Hayashi*, "Supramolecular Hemoprotein Assembly with a Periodic Structure Showing Heme–Heme Exciton Coupling", Journal of American Chemical Society 140, 10145-10148 (2018). DOI: 10.1021/jacs.8b06690

Tetsuya Mori#, Shogo Sugiyama#, Mark Byne, Carl Hirschie Johnson*, Takayuki Uchihashi* and Toshio Ando*, "Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time", Nature Communications 9, Article number: 3245 (2018). (#equal contribution) DOI: 10.1038/s41467-018-05438-4

Takayuki Uchihashi*, Hiroki Watanabe and Noriyuki Kodera, "Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy", Methods in Molecular Biology 1814, 159-179 (2018). DOI: 10.1007/978-1-4939-8591-3_10

Masanori Hosoyamada, Nobuhiro Yanai*, Keisuke Okumura, Takayuki Uchihashi and Nobuo Kimizuka*, "Translating MOF chemistry into supramolecular chemistry: soluble coordination nanofibers showing efficient photon upconversion", Chemical Communications 54, 6828-6831 (2018) DOI: 10.1039/c8cc01594e

Takayuki Uchihashi#, Yo-hei Watanabe#*, Yosuke Nakazaki, Takashi Yamasaki, Hiroki Watanabe, Takahiro Maruno, Kentaro Ishii, Susumu Uchiyama, Chihong Song, Kazuyoshi Murata, Ryota Iino*, Toshio Ando*, "Dynamic structural states of ClpB involved in its disaggregation function", Nature Communications 9, Article number: 2147 (2018). (#equal contribution) DOI: 10.1038/s41467-018-04587-w

Mikihiro Shibata#, Keiichi Inoue#, Kento Ikeda, Masae Konno, Manish Singh, Chihiro Kataoka, Rei Abe-Yoshizumi, Hideki Kandori*, Takayuki Uchihashi*, "Oligomeric states of microbial rhodopsins determined by high-speed atomic force microscopy and circular dichroic spectroscopy", Scientific Reports 8, Article number: 8262 (2018). (#equal contribution) DOI:  10.1038/s41598-018-26606-y

Takahiro Maruno, Hiroki Watanabe, Takayuki Uchihashi, Satoru Adachi, Kunihito Arai, Taichi Sawaguchi and Susumu Uchiyama*, "Sweeping of adsorbed therapeutic proteins on prefillable syringe enhances subvisible particles generation", Journal of Pharmaceutical Sciences 107, pp. 1521-1529 (2018). DOI: 10.1016/j.xphs.2018.01.021

Takayuki Umakoshi, Hikari Udaka, Takayuki Uchihashi, Toshio Ando, Miho Suzuki, Takeshi Fukuda*, "Quantum-dot antibody conjugation visualized at the single-molecule scale with high-speed atomic force microscopy", Colloids and Surfaces B: Biointerfaces 167, pp. 264-274 (2018). DOI: 10.1016/j.colsurfb.2018.04.015

Naoya Terahara, Yumi Inoue, Noriyuki Kodera, Yusuke V. Morimoto, Takayuki Uchihashi, Katsumi Imada, Toshio Ando, Keiichi Namba, and Thoru Minamino*, "Insight into structural remodeling of the FlhA ring responsible for bacterial flagellar type III protein export", Science Advances 4, eaao7054 (2018). DOI: 10.1126/sciadv.aao7054

Hisao Tsukamoto, Masahiro Higashi, Hideyoshi Motoki, Hiroki Watanabe, Christian Ganser, Koichi Nakajo, Yoshihiro Kubo, Takayuki Uchihashi and Yuji Furutani*, "Structural properties determining low K+ affinity of the selectivity filter in the TWIK1 K+ channel", Journal of Biological Chemistry 293, pp. 6969-6984 (2018). DOI: 10.1074/jbc.RA118.001817

Akiya Oda, Satoshi Nagao, Masaru Yamanaka, Hiroki Watanabe, Takayuki Uchihashi, Ikki Ueda, Naoki Shibata, Yoshiki Higuchi, and Shun Hirota*, "Construction of a Triangle-Shaped Trimer and a Tetrahedral Structure Using an α-Helix-Inserted Circular Permutant of Cytochrome c555", Chemistry - An Asian Journal 13, pp. 964-967 (2018). DOI: 10.1002/asia.201800252

Takamitsu Haruyama, Takayuki Uchihashi, Yutaro Yamada, Noriyuki Kodera, Toshio Ando and Hiroki Konno*, "Negatively charged lipids are essential for functional and structural switch of human 2-Cys peroxiredoxin II", Journal of Molecular Biology 430, pp. 602-610 (2018). DOI: 10.1016/j.jmb.2017.12.020

Tetsuya Takeda*, Toshiya Kozai, Huiran Yang, Daiki Ishikuro, Kaho Seyama, Yusuke Kumagai, Tadashi Abe, Hiroshi Yamada, Takayuki Uchihashi, Toshio Ando* and Kohji Takei*, "Dynamic clustering of dynamin-amphiphysin helices regulates membrane constriction and fission coupled with GTP hydrolysis", Elife 7, e3024 (2018). DOI: 10.7554/eLife.30246

Maho Yagi-Utsumi, Arunima Sikdar, Toshiya Kozai, Rintaro Inoue, Masaaki Sugiyama, Takayuki Uchihashi, Tadashi Satoh and Koichi Kato*, "Conversion of functionally undefined homopentameric protein PbaA into a proteasome activator by mutational modification of its C-terminal segment conformation", Protein engineering design & selection 31, 29-36 (2018). DOI: 10.1093/protein/gzx066

Akihiko Nakamura, Tomoyuki Tasaki, Yasuko Okuni, Chihong Song, Kazuyoshi Murata, Toshiya Kozai, Mayu Hara, Hayuki Sugimoto, Kazushi Suzuki, Takeshi Watanabe, Takayuki Uchihashi, Hiroyuki Noji and Ryota Iino*, "Rate constants, processivity, and productive binding ratio of chitinase A revealed by single-molecule analysis", Physical Chemistry Chemical Physics 20, 3010-3018 (2018). DOI: 10.1039/c7cp04606e

Takayuki Uchihashi* and Simon Scheuring, "Review: Applications of high-speed atomic force microscopy to real-time visualization of dynamic biomolecular processes", BBA General Subjects 1862, 229-240 (2018). DOI: 10.1016/j.bbagen.2017.07.010

2018

2017

Toshiya Kozai, Taichiro Sekiguchi, Tadashi Satoh, Hirokazu Yagi, Koichi Kato * and Takayuki Uchihashi *, "Two-step process for disassembly mechanism of proteasome α7 homo-tetradecamer by α6 revealed by high-speed atomic force microscopy", Scientific Reports 7, article number: 15373 (2017). DOI: 10.1038 / s41598-017-15708-8.

Naoya Terahara, Noriyuki Kodera, Takayuki Uchihashi, Toshio Ando, Keiichi Namba * and Tohru Minamino *, "Na + -induced structural transition of MotPS for stator assembly of Bacillus flagellar motor", Science Advances 3, eaao4119 (2017). DOI: 10.1126 / sciadv.aao4119.

Mikihiro Shibata *, Hiroki Watanabe, Takayuki Uchihashi, Toshio Ando, and Ryohei Yasuda, "High-speed atomic force microscopy imaging of live mammalian cells", Biophysics and Physicobiology 14, 127-135 (2017). DOI: 10.2142 / biophysico.14.0 _127

Mikihiro Shibata *, Hiroshi Nishimasu *, Noriyuki Kodera, Seiichi Hirano, Toshio Ando, Takayuki Uchihashi and Osamu Nureki *, "Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy", Nature Communications 8 , article number: 1430 (2017). DOI: 10.1038 / s41467-017-01466-8.

Tadashi Satoh *, Chihong Song, Tong Zhu, Takayasu Toshimori, Kazuyoshi Murata, Yugo Hayashi, Hironari Kamikubo, Takayuki Uchihashi and Koichi Kato *, "Visualisation of a flexible modular structure of the ER folding-sensor enzyme UGGT", Scientific Reports 7, Article Number: 12142 (2017). DOI: 10.1038 / s41598-017-12283-w.

Hirofumi Harada, Akira Onoda *, Takayuki Uchihashi *, Hiroki Watanabe, Naoki Sunagawa, Masahiro Samejima, Kiyohiko Igarashi *, and Takashi Hayashi, "Interdomain Flip-flop Motion Visualized in Flavocytochrome Cellobiose Dehydrogenase Using High-speed Atomic Force Microscopy during Catalysis", Chemical Science 8, 6561-6565 (2017). DOI: 10.1039 / c7sc01672g.

Shusuke Matsui, Takuma Kureha, Seina Hiroshige, Mikihiro Shibata, Takayuki Uchihashi *, and Daisuke Suzuki *, "Fast Adsorption of Soft Hydrogel Microspheres on Solid Surfaces in Aqueous Solution", Angewandte Chemie International Edition (Communication) 56, 12146-12149 (2017) ). DOI: 10.1002 / anie.201705808.

Jakia Jannat Keya, Daisuke Inoue, Yuki Suzuki, Toshiya Kozai, Daiki Ishikuro, Noriyuki Kodera, Takayuki Uchihashi, Arif Md. Rashedul Kabir, Masayuki Endo, Kazuki Sada, Akira Kakugo, "High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by" HS-AFM ", Scientific Reports 7, 6166 (2017). DOI: 10.1038 / s41598-017-06249-1.

Mahmoud Mohamed, Akiko Kobayashi, Azuma Taoka, Takahiro Watanabe-Nakayama, Yosuke Kikuchi, Masaharu Hazawa, Toshinari Minamoto, Yoshihiro Fukumori, Noriyuki Kodera, Takayuki Uchihashi, Toshio Ando and Richard Wong *, "High-Speed Atomic Force Microscopy Reveals Loss of Nuclear" Pore Resilience as a Dying Code in Colorectal Cancer Cells ", ACS Nano 11, 5567-5578 (2017). DOI: 10.1021 / acsnano.7b00906.

Ayumi Sumino, Takayuki Uchihashi and Shigetoshi Oiki *, "Oriented Reconstitution of the Full-Length KcsA Potassium Channel in a Lipid Bilayer for AFM Imaging", J. Phy. Chem. Lett. 8, 785-793 (2017). DOI: 10.1021 /acs.jpclett.6b03058.

2017
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